elec. transfer of DNA from yeast to E. coli

fleisch at embratel.net.br fleisch at embratel.net.br
Wed Sep 20 11:55:34 EST 1995


Hello netters,
   I use the plasmid isolation with glass beads and the transformation by 
electroporation protocols cited in "Cunts protocols in molecular genetics" 
(Ausubel et al 1991). They use to work well. If you don't get transformants 
try to isolate the plasmids with a better protocol (maybe the plasmids have to 
be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
  I hope it works.
                          Claus

                               



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