elec. transfer of DNA from yeast to E. coli
chester.cooper at utmb.edu
Wed Sep 20 12:44:36 EST 1995
Re: Claus' recent message:
> I use the plasmid isolation with glass beads and the transformation by
>electroporation protocols cited in "Cunts protocols in molecular genetics"
>(Ausubel et al 1991). They use to work well. If you don't get transformants
>try to isolate the plasmids with a better protocol (maybe the plasmids have to
>be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
> I hope it works.
So Claus, exactly which adult book store carries Aususbel et al's book and
where is it located. We are all anxious to get a copy of it.
Chet Cooper Facsimile: (409) 747-2437
Department of Pathology Office: (409) 747-2438
University of Texas Medical Branch Laboratory: (409) 747-2439
301 University Boulevard Admin. Asst.: (409) 747-0604
Galveston, Texas 77555-0609 E-Mail: CRCOOPER at beach.utmb.edu
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