trp1-289 reversion help
shin at biosci.cbs.umn.edu
Thu Sep 21 06:20:33 EST 1995
I am trying to revert trp1-289 in budding yeast strain S150B2 using a TRP1
clone by transformation.
I used the EcoRI-BglII fragment to avoid the ARS1 for the transformation
but I keep getting the TRP+ transformants that behave as plasmid
transformants. I am guessing that a TRP1-ARS1 circle is forming somehow,
since the plasmid tranformants appear to be relatively stable.
Is there a trick that I can use to avoid this? I have a mutant that I
suspect is linked to TRP1 and would like to show this without outcrossing
to another background.
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