ADE2 red pigment.

[Tomas Drgon]

In article <43q4sd$1do at urca.nce.ufrj.br>, egbjoab at vmhpo.uff.br wrote:

> Hi:
>         I'd like to get a protocol to quantifie the red pigment
> accumalated by the ade2 mutants of Saccharomyces cerevisiae.
>         I will appreciate any help.

The pigment is fluorescent (the same wavelenght as quinacrine). You can do
direct fluorescence of cell suspension. I can try to dig out the protocol,
but I'm not sure, because it was a couple of years ago.
BTW, it accumulates in vacuoles, you can see stained vacuoles in FM. This
ade2 is simply cool.   :-)

Tomas

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