ADE2 red pigment.

Tomas Drgon tomasd at
Thu Sep 21 09:32:52 EST 1995

In article <43q4sd$1do at>, egbjoab at wrote:

> Hi:
>         I'd like to get a protocol to quantifie the red pigment
> accumalated by the ade2 mutants of Saccharomyces cerevisiae.
>         I will appreciate any help.

The pigment is fluorescent (the same wavelenght as quinacrine). You can do
direct fluorescence of cell suspension. I can try to dig out the protocol,
but I'm not sure, because it was a couple of years ago.
BTW, it accumulates in vacuoles, you can see stained vacuoles in FM. This
ade2 is simply cool.   :-)


Vidno jak psoj kulky.
(Old Slovak proverb)

More information about the Yeast mailing list