elec. transfer of DNA from yeast to E. coli

BRAINSL at aol.com BRAINSL at aol.com
Mon Sep 25 08:05:37 EST 1995


This email was sent to us in error.  This email must belongs to
BRIANSL at aol.com.    BRIAN is a guys NAME!

We are BRAINSL (as in the human mind)  Please take us off your email listing.
 Thank you.

===================================================


In a message dated 95-09-20 14:40:57 EDT, BIOSCI-REQUEST at net.bio.net writes:

>Subj:	RE: elec. transfer of DNA from yeast to E. coli
>Date:	95-09-20 14:40:57 EDT
>From:	chester.cooper at utmb.edu (Chester Cooper)
>Sender:	daemon at net.bio.net
>Resent-from:	BIOSCI-REQUEST at net.bio.net
>To:	yeast at net.bio.net
>
>Re: Claus' recent message:
>
>>Hello netters,
>>   I use the plasmid isolation with glass beads and the transformation by 
>>electroporation protocols cited in "Cunts protocols in molecular genetics" 
>>(Ausubel et al 1991). They use to work well. If you don't get transformants

>>try to isolate the plasmids with a better protocol (maybe the plasmids have
>to 
>>be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
>>  I hope it works.
>>                          Claus
>>
>>                               
>So Claus, exactly which adult book store carries Aususbel et al's book and
>where is it located.  We are all anxious to get a copy of it.
>
>
>Chet Cooper                              Facsimile:    (409) 747-2437
>Department of Pathology                  Office:       (409) 747-2438
>University of Texas Medical Branch       Laboratory:   (409) 747-2439
>301 University Boulevard                 Admin. Asst.: (409) 747-0604
>Galveston, Texas 77555-0609              E-Mail: CRCOOPER at beach.utmb.edu   
>          
>
>
>
>
>
>----------------------- Headers --------------------------------
>From BIOSCI-REQUEST at net.bio.net  Wed Sep 20 14:20:01 1995
>Return-Path: BIOSCI-REQUEST at net.bio.net
>Received: from OCELOT.RUTGERS.EDU (ocelot.rutgers.edu [128.6.11.33]) by
>emin01.mail.aol.com (8.6.12/8.6.12) with SMTP id OAA07264 for
><brainsl at aol.com>; Wed, 20 Sep 1995 14:19:59 -0400
>Resent-From: BIOSCI-REQUEST at net.bio.net
>Received: from mbcl.rutgers.edu by mbcl.rutgers.edu (PMDF #12194) id
> <01HVI2ZAZ4V4A9MFWY at mbcl.rutgers.edu>; Wed, 20 Sep 1995 14:15 EDT
>Received: from net.bio.net by mbcl.rutgers.edu (PMDF #12194) id
> <01HVI2YES4BK9TF643 at mbcl.rutgers.edu>; Wed, 20 Sep 1995 14:14 EDT
>Received: (from daemon at localhost) by net.bio.net (8.6.12/8.6.6) id KAA24072
>for
> yeast-list; Wed, 20 Sep 1995 10:44:42 -0700
>Received: (from news at localhost) by net.bio.net (8.6.12/8.6.6) id KAA24067
for
> yeast-arpanet; Wed, 20 Sep 1995 10:44:41 -0700
>Resent-date: Wed, 20 Sep 1995 14:15 EDT
>Date: 20 Sep 1995 10:44:36 -0700
>From: chester.cooper at utmb.edu (Chester Cooper)
>Subject: RE: elec. transfer of DNA from yeast to E. coli
>Sender: daemon at net.bio.net
>Resent-to: brainsl at aol.com,
>        Neigeborn#m#_Lenore.mbcl_microsoft_mailserver at raven.rutgers.edu,
>        Vershon#m#_Andrew_K.mbcl_microsoft_mailserver at raven.rutgers.edu
>To: yeast at net.bio.net
>Resent-message-id: <01HVI2ZAZ4V4A9MFWY at mbcl.rutgers.edu>
>Message-id: <01HVHZO0YMMA002DD9 at BEACH.UTMB.EDU>
>X-Envelope-to: brainsl at aol.com
>X-VMS-To: IN%"yeast at net.bio.net"
>NNTP-Posting-Host: net.bio.net





More information about the Yeast mailing list