elec. transfer of DNA from yeast to E. coli
BRAINSL at aol.com
BRAINSL at aol.com
Mon Sep 25 08:05:37 EST 1995
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We are BRAINSL (as in the human mind) Please take us off your email listing.
Thank you.
===================================================
In a message dated 95-09-20 14:40:57 EDT, BIOSCI-REQUEST at net.bio.net writes:
>Subj: RE: elec. transfer of DNA from yeast to E. coli
>Date: 95-09-20 14:40:57 EDT
>From: chester.cooper at utmb.edu (Chester Cooper)
>Sender: daemon at net.bio.net
>Resent-from: BIOSCI-REQUEST at net.bio.net
>To: yeast at net.bio.net
>
>Re: Claus' recent message:
>
>>Hello netters,
>> I use the plasmid isolation with glass beads and the transformation by
>>electroporation protocols cited in "Cunts protocols in molecular genetics"
>>(Ausubel et al 1991). They use to work well. If you don't get transformants
>>try to isolate the plasmids with a better protocol (maybe the plasmids have
>to
>>be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
>> I hope it works.
>> Claus
>>
>>
>So Claus, exactly which adult book store carries Aususbel et al's book and
>where is it located. We are all anxious to get a copy of it.
>
>
>Chet Cooper Facsimile: (409) 747-2437
>Department of Pathology Office: (409) 747-2438
>University of Texas Medical Branch Laboratory: (409) 747-2439
>301 University Boulevard Admin. Asst.: (409) 747-0604
>Galveston, Texas 77555-0609 E-Mail: CRCOOPER at beach.utmb.edu
>
>
>
>
>
>
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>From: chester.cooper at utmb.edu (Chester Cooper)
>Subject: RE: elec. transfer of DNA from yeast to E. coli
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