elec. transfer of DNA from yeast to E. coli

BRAINSL at aol.com BRAINSL at aol.com
Mon Sep 25 08:04:19 EST 1995


In a message dated 95-09-20 13:52:24 EDT, BIOSCI-REQUEST at net.bio.net writes:

>Subj:	RE: elec. transfer of DNA from yeast to E. coli
>Date:	95-09-20 13:52:24 EDT
>From:	fleisch at embratel.net.br
>Sender:	daemon at net.bio.net
>Resent-from:	BIOSCI-REQUEST at net.bio.net
>To:	yeast at net.bio.net
>
>Hello netters,
>   I use the plasmid isolation with glass beads and the transformation by 
>electroporation protocols cited in "Cunts protocols in molecular genetics" 
>(Ausubel et al 1991). They use to work well. If you don't get transformants 
>try to isolate the plasmids with a better protocol (maybe the plasmids have
>to 
>be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
>  I hope it works.
>                          Claus
>
>                               
>
>
>
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>Date: 20 Sep 1995 09:55:34 -0700
>From: fleisch at embratel.net.br
>Subject: RE: elec. transfer of DNA from yeast to E. coli
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