glass beads for yeast lysis

BRAINSL at aol.com BRAINSL at aol.com
Mon Sep 25 07:43:45 EST 1995


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In a message dated 95-09-19 21:55:21 EDT, BIOSCI-REQUEST at net.bio.net writes:

>Subj:	glass beads for yeast lysis
>Date:	95-09-19 21:55:21 EDT
>From:	bicfo at flinders.edu.au
>Resent-from:	BIOSCI-REQUEST at net.bio.net
>To:	yeast at net.bio.net
>
>hallo all!
>Can someone give me the good oil on using glass beads to lyse yeast? I'm
>using 
>1ml of lysis buffer (50mM HEPES, pH 7.4, 150mM NaCl, 5mM EGTA, 5mM EDTA, 2% 
>Triton x-100 and inhibitors) to ~ 6 x 10^8 cells and 1.5g 400 -600u 
>acid-washed beads. I'm vortexing them to death - and yet I'm getting zilch
in
>
>the way of lysis! What am I failing to do?
>Replies gratefully received!
>Cheers, Colleen
>
>
>
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>Date: Tue, 19 Sep 1995 05:53:11 GMT
>From: bicfo at flinders.edu.au
>Subject: glass beads for yeast lysis
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