halting transcription?

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Mon Sep 25 07:52:16 EST 1995


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In a message dated 95-09-20 06:31:31 EDT, BIOSCI-REQUEST at net.bio.net writes:

>Subj:	RE: halting transcription?
>Date:	95-09-20 06:31:31 EDT
>From:	rdlab at gandalf.bio.uci.edu (rlab davis)
>Resent-from:	BIOSCI-REQUEST at net.bio.net
>To:	yeast at net.bio.net
>
>In article lgood at uoguelph.ca (Liam E Good) writes:
>
>>I would like to halt transcription in S. pombe cells.  I think that this 
>>can be done easily in vitro using Actinomycin D, but I am not 
>>sure that the drug will enter yeast cells and work efficiently in 
>>vivo.  Does anyone have any comments or alternatives?
>
>Thiolutin has been used by Allan Jacobsen's lab, and others to inhibit 
>transcription in S. cerevisiae (see Herrick, et al., 1990, Mol. Cell. Biol. 
>10: 2269-2284).  We have tried it, and found it effective in Neurospora 
>crassa, whereas Actinomycin D was not, so it might work in S. pombe. 
>A major drawback is that it is not commericially available (see Herrick
>et al. for a possible source), and it also has inhibitory effects on trans-
>lation.
>
>Martin Hoyt (R H Davis lab)
>Dept. Mol. Biol. and Biochem.
>University of California, Irvine
>mahoyt at uci.edu
>
>
>
>
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>Date: Tue, 19 Sep 1995 20:51:59
>From: rdlab at gandalf.bio.uci.edu (rlab davis)
>Subject: RE: halting transcription?
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