elec. transfer of DNA from yeast to E. coli

BRAINSL at aol.com BRAINSL at aol.com
Mon Sep 25 08:29:48 EST 1995


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In a message dated 95-09-25 09:17:37 EDT, BIOSCI-REQUEST at net.bio.net writes:

>Subj:	RE: elec. transfer of DNA from yeast to E. coli
>Date:	95-09-25 09:17:37 EDT
>Sender:	daemon at net.bio.net
>Resent-from:	BIOSCI-REQUEST at net.bio.net
>To:	yeast at net.bio.net
>
>This email was sent to us in error.  This email must belongs to
>BRIANSL at aol.com.    BRIAN is a guys NAME!
>
>We are BRAINSL (as in the human mind)  Please take us off your email
listing.
> Thank you.
>
>===================================================
>
>
>In a message dated 95-09-20 14:40:57 EDT, BIOSCI-REQUEST at net.bio.net writes:
>
>>Subj:	RE: elec. transfer of DNA from yeast to E. coli
>>Date:	95-09-20 14:40:57 EDT
>>From:	chester.cooper at utmb.edu (Chester Cooper)
>>Sender:	daemon at net.bio.net
>>Resent-from:	BIOSCI-REQUEST at net.bio.net
>>To:	yeast at net.bio.net
>>
>>Re: Claus' recent message:
>>
>>>Hello netters,
>>>   I use the plasmid isolation with glass beads and the transformation by 
>>>electroporation protocols cited in "Cunts protocols in molecular genetics"

>>>(Ausubel et al 1991). They use to work well. If you don't get
transformants
>
>>>try to isolate the plasmids with a better protocol (maybe the plasmids
have
>>to 
>>>be cleaner). For this I sugest Holm et al (1986) Gene, 42: 169-173.
>>>  I hope it works.
>>>                          Claus
>>>
>>>                               
>>So Claus, exactly which adult book store carries Aususbel et al's book and
>>where is it located.  We are all anxious to get a copy of it.
>>
>>
>>Chet Cooper                              Facsimile:    (409) 747-2437
>>Department of Pathology                  Office:       (409) 747-2438
>>University of Texas Medical Branch       Laboratory:   (409) 747-2439
>>301 University Boulevard                 Admin. Asst.: (409) 747-0604
>>Galveston, Texas 77555-0609              E-Mail: CRCOOPER at beach.utmb.edu   
>>          
>>
>>
>>
>>
>>
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>>Subject: RE: elec. transfer of DNA from yeast to E. coli
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>Date: 25 Sep 1995 06:05:37 -0700
>From: BRAINSL at aol.com
>Subject: RE: elec. transfer of DNA from yeast to E. coli
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