Allen.Donald at BBSRC.AC.UK
Thu Sep 28 08:57:54 EST 1995
In my experience it is a question of getting the balance between a firm
enough pellet (so you don't lose all the protoplasts), and the possibilty
of breaking the protoplasts. I would say somewhere between 2000-3000g,
for about 5 minutes is about right.
I used to find the protoplasts were quite hard to resuspend after
treatment but be patient!
More information about the Yeast