colony hybridization?

Patrick Hurban hurban at cmgm.stanford.edu
Mon Apr 1 18:19:46 EST 1996


Dear Yeast Experts,

I am in a fly lab but find myself working with yeast as part of a 2-hybrid
screen.  I would like to know if there are any protocols out there to
perform a colony hybridization, aka "Grunstein-Hogness Hybridization", on
yeast colonies instead of bacteria.  I haven't seen any and I'm guessing
that the cell wall may be a problem in this regard but I thought it best
to ask the experts directly.  Please reply with any information you may
have.  Thank you in advance.

-- 
Ciao for now,

Patrick Hurban
Department of Developmental Biology
Beckman Center B361
Stanford University School of Medicine
Stanford  CA  94305



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