colony hybridization?

Michael Lichten lichten at helix.nih.gov
Tue Apr 2 12:16:50 EST 1996


In article
<hurban-0104961519460001 at beckman-42-fp-dynamic-164.stanford.edu>,
hurban at cmgm.stanford.edu (Patrick Hurban) wrote:

> Dear Yeast Experts,
> 
> I am in a fly lab but find myself working with yeast as part of a 2-hybrid
> screen.  I would like to know if there are any protocols out there to
> perform a colony hybridization, aka "Grunstein-Hogness Hybridization", on
> yeast colonies instead of bacteria.  I haven't seen any and I'm guessing
> that the cell wall may be a problem in this regard but I thought it best
> to ask the experts directly.  Please reply with any information you may
> have.  Thank you in advance.
> 
> -- 
> Ciao for now,
> 
> Patrick Hurban
> Department of Developmental Biology
> Beckman Center B361
> Stanford University School of Medicine
> Stanford  CA  94305

There is a protocol in Methods in Yeast Genetics (1990) Rose, Winston, and
Hieter, Cold Spring Harbor Laboratory Press.  I have had variable results
with it--so it's important to have + and - controls (as if it weren't
always important....).

-- 
Michael Lichten
lichten at helix.nih.gov



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