Are they true positves?
sltan at u.washington.edu
Thu Apr 4 18:14:26 EST 1996
I've just done a yeast 2-hybrid screen using the Clontech Matchmaker kit.
I made my own library using Stratagene HybridZap kit. I had some
pink colonies that grew on -His plates but they looked weird or different
from positve control Hf7c strain containing P53 and T Ag. They also didn't
smell right. They, nevertheless, turned blue on beta-gal lift assays.
Although, I was very careful when plating my cells, I now wonder if my
colonies are contaminants. Are there any bacteria or any other bugs that
can grow on -His plates, look pink and use X-gal as subtrate?
Please help. Many thanks in advance.
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