cyc minimal LacZ reporter plasmids
Detlef_Weigel at QM.SALK.EDU
Thu Apr 4 17:25:41 EST 1996
Hello Everybody out there,
I am looking for a yeast reporter plasmid having (if possible) a
multicloning site at the 5' end of the minimal CYC promoter driving
LacZ ("Guarente type" plasmids) but having either a TRP1 or a HIS3
marker (my experimental setup is such that the LEU2 and URA3 markers
are already taken by other plasmids).
If on top of that anybody would have a similar plasmid but driving
the HIS3 gene instead of the LacZ gene (this would obviously have to
be a TRP1 based plasmid...) to make some one hybrid type of approach
it would be just perfect.
Thanks a lot for your help
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