brennan at toxsparc.harvard.edu
Thu Apr 11 12:46:13 EST 1996
I also heard the calcium theory from an old prof, you might try EGTA at ~1mM.
Alternatively 50microL tween 80 per L medium can help.
brennan at mbcrr.harvard.edu
Michael Lichten (lichten at helix.nih.gov) wrote:
: In article <316C76CE.5798 at powergrid.electriciti.com>,
: dhiggins at powergrid.electriciti.com wrote:
: > Are there any innocuous compounds that you can add to a yeast culture
: > that will reduce or eliminate flocculation? Thanks.
: I have been told that some flocculation is calcium dependent. We have
: found that for the clumping that strain SK1 displays, the addition of
: polypropylene glycol 2000 at 10 ppm (i.e. 0.001%) helps considerably to
: reduce clumping, although it doesn't eliminate it. SK1 doesn't really
: clump much until it stops fermenting; this is of course no help if you
: have to grow high density cultures.
: Michael Lichten
: lichten at helix.nih.gov
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