5-FOA and S.pombe

Hoffmacs Hoffmacs at HERMES.BC.EDU
Sat Apr 13 08:29:47 EST 1996


Dear Emma et al.,
I have used 5FOA to integrate reporter fusions into ura4 using the same
recipe as for S. cerevisiae (the YNB-aa-am. sulfate recipe).  It should work.
 Good luck.
Charlie Hoffman
"I'd rather be fission."
________________________________________________________

Can anyone tell me what the working concentration is of 5-FOA for 
S.pombe.......for S.cerivisiae it's 1mg/ml of media.
Any other 'top tips' / 'tricks of the trade' when selecting with 5-FOA 
would also be really useful as this is my first time using it.  
Thanks in advance.

Emma Tonkin
Dept. Molecular and Cell Biology
University of Aberdeen
Scotland
Fax: (01224) 273144
email: gen160 at abdn.ac.uk





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