help - yeast plasmid rapid miniprep needed!

Stephan Witte stephan.witte at uni-konstanz.de
Sun Apr 14 05:04:41 EST 1996


Hi netters!

					After a two hybrid/interaction trap screen I have to isolate plasmids
from my positive clones.
I didnt succeed with a zymolase protocol, I suppose the enzyme does not
lyse the cells.
Are there other reliable protocols, maybe something like alkaline
lysis...... (not much YEAST experience :-()

Thanks a lot,

Stephan 

*******************************************
Stephan Witte
Inst. of Immunology
University of Konstanz
Postfach 5560 M 662
78434 Konstanz
Germany

Phone: +49-7531-88-2749
Fax:     +49-7531-88-3102
e-mail: stephan.witte at uni-konstanz.de
*******************************************



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