Strain Pb, Uracile requirement

Guillaume Stahl stahl at infobiogen.fr
Mon Apr 22 09:30:54 EST 1996


Hi Yeastnetters,

Perhaps someone may help me : I'm working with an unknown yeast 
strain, which name is YBAM2 5A, and I think is from a YPH250 strain.
This strain requires more uracile in minimal medium to grow (around 5 
times ?). Usual concentration used in the lab is 4 mg/100ml. Cells 
stop growing around 0.2 OD600 in those conditions. Does anyones has 
ever observed this, and may recommend me specific media to use with 
this strain.
----------------------------------------------------------------------
Guillaume Stahl                            Tel.    (33 1) 69 41 72 64
Institut de Genetique et Microbiologie     Fax.    (33 1) 69 41 72 96
batiment 400, Universite Paris-Sud         e-mail stahl at infobiogen.fr
F-91405 Orsay Cedex, France



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