Sanford Silverman silvermans at
Tue Apr 23 19:01:28 EST 1996

Karen Yoas wrote:
> I have been told that Calcofluor stains bud sites in S. cerevisiae.
> Are there structural differences which allow for staining only the bud
> site
> or is it something else?
> Karen Yoas
> Biological & Biochemical Sciences
> University of Houston
> Houston, TX 77204-5934

Calcofluor is not very specific for polysaccharide staining, but in Saccharomyces cerevisiae it 
essentially stains only chitin, which appears in the mother/bud neck region.
Sanford Silverman               >Opinions expressed here are my own< 
American Cyanamid  
silvermans at                      "Yeast is Best"

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