seeking immunofluorescence technique

[Sepp D. Kohlwein]

debbritt at brownvm.brown.edu (Deb Britt) wrote:
>I'm looking for a technique to perform indirect immunofluorescence to
>detect production of a particular protein in yeast cells.  I did find some
>references through a medline search, but I would be interested in hearing
>some recommendations from people as to what protocols they use to get
>reliable results.  You can post or email me directly.  Thanks!
>
>Deb
>
>-- 
>Deborah Britt, Ph.D.
>Medical Oncology
>RI Hospital/Brown University

Hi Deborah,

A good start would be Pringle et al. in Methods in Cell Biology, Vol 31 Chapter 19, pp357-435 (Fluorescence microscopy methods for yeast).

Good luck !

Sepp D. Kohlwein


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Sepp D. Kohlwein, PhD              
Genetics and Molecular Biology Group
Department of Biochemistry and Food Chemistry
Technical University Graz                   phone:  ++43 (316) 873-6456
Petersgasse 12                              fax:    ++43 (316) 873-6952
A 8010 Graz, Austria               e-mail kohlwein at ftugax.tu-graz.ac.at
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