COMPLEMENTATIONOF A SLOW GROWING STRAIN

[Guillaume Stahl]

Hi,
I don't know if this answers the question, but you may try the plasmid 
shuffling experiment : your disrupted/mutated gene is on an episome 
which you may counter-select (URA3...). This leads to a good growing 
strain, and may be transformed as you like. Thereafter you may counter 
select your first plasmid (5-FOA), and fish a complementing gene...

To resume : transform your slow growing yeast into a rapid growing 
yeast and use the LiAc method, which seems to be the best one.

Hope this helps

Guillaume Stahl, France