COMPLEMENTATIONOF A SLOW GROWING STRAIN

Guillaume Stahl stahl at infobiogen.fr
Fri Apr 26 05:47:57 EST 1996


Hi,
I don't know if this answers the question, but you may try the plasmid 
shuffling experiment : your disrupted/mutated gene is on an episome 
which you may counter-select (URA3...). This leads to a good growing 
strain, and may be transformed as you like. Thereafter you may counter 
select your first plasmid (5-FOA), and fish a complementing gene...

To resume : transform your slow growing yeast into a rapid growing 
yeast and use the LiAc method, which seems to be the best one.

Hope this helps

Guillaume Stahl, France



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