FOA

[Francisco G da Nobrega ib - bio 7588]

Dear Drgon:

Working with yeast strain W303 I noticed that there is always 
considerable background growth when cells are plated on minimal FOA 
medium plus uracil. If you plate too few cells it might be difficult to 
pick a good crop of FOA resistant (ura3-) cells but they will come out 
very clear. If more cells are plated you must wait up to 1 week to pick 
the colonies that rise above the background, repurify them and re test on 
minimal - uracil. This residual growth might be starin dependent.

F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900   Sao Paulo  -  SP  - Brasil
Voice: (011) 818-7588    Fax: 55-11-818-7354    E-mail:  fgdnobre at usp.br

On Thu, 1 Aug 1996, Tomas Drgon wrote:

> Hi folks.
> I have a question: How many cells can you plate on FOA plate and still get
> selection? Is there an upper limit?
> 
> Tomas Drgon
> 
> -- 
> Vinarske zavody Pezinok pozdravuju vsetky pokrokove sily sveta!
> 
>