yeast PCR

klennon at acs.bu.edu klennon at acs.bu.edu
Thu Aug 8 12:34:53 EST 1996


 Hi.

I am currently in the process of setting up the Two Hybrid System. I expressed 
my BD-protein of interest in strain CG1954 and want to do PCR directly from 
colonies. The question I have is:  how can I lyse the cells so that I can do 
PCR directly (without having to do plasmid isolation first and then PCR)? High 
temp or low temp? Neither?

Any advice you could give would be greatly appreciated.

Thanks

Kelley and Iveta

(please email responses to iiontche at acs.bu.edu)



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