yeast PCR

klennon at klennon at
Thu Aug 8 12:34:53 EST 1996


I am currently in the process of setting up the Two Hybrid System. I expressed 
my BD-protein of interest in strain CG1954 and want to do PCR directly from 
colonies. The question I have is:  how can I lyse the cells so that I can do 
PCR directly (without having to do plasmid isolation first and then PCR)? High 
temp or low temp? Neither?

Any advice you could give would be greatly appreciated.


Kelley and Iveta

(please email responses to iiontche at

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