Addition of CaCl2 into YPD

Tang Hsin Yao mcbthy at
Fri Aug 9 21:04:29 EST 1996

Hi! Does anyone know how to add Calcium Chloride (CaCl2) into YPD? I have 
tried to make YPD plates supplemented with 100mM CaCl2. I first dissolved 
the CaCl2, then I added the usual stuff for making YPD. But after I 
autoclaved the solution, the CaCl2 seems to ppt out. Do I need to adjust 
the pH or something? I have done the same thing with MgCl2 and there was 
no problem. Please advice me on what to do. Thank you very much for your 
kind attention.

Hsinyao Tang
mcbthy at

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