plasmids

Solange S de Camargo scamargo at usp.br
Mon Aug 12 10:51:36 EST 1996


Hi folks, 

	Thanks for all that replied my message about geneticin. It really 
worked out. One of the strains was sensitive to 50ug/ml and the other to 
100ug/ml in liquid YPD medium. I think it's ok and I intend to analyze 
them in solid medium before the transformation.
	Now I have another question: a colleague of mine wants to 
do a plasmid extraction directly from yeast without the step that 
involves bacteria transformation. Does anybody know about any method? She 
can't select the gene she is cloning in bacteria because it is letal for 
this organism. If somebody could help us, we'd appreciate a lot.
	Thanks in advance,
	Solange  



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