Yeast Two Hybrid : PCR of Inserts in pGAD10
neil at GEN.MPIB-TUEBINGEN.MPG.DE
Wed Aug 21 07:33:26 EST 1996
We are currently using both the Brent and Clontech Matchmaker yeast
two-hybrid systems and are trying to amplify inserts in selected
pGAD10 library members with very little success.
Does anyone have working primer sequences and/or conditions to amplify
sequences from yeast minis....?
Thanks in advance
(neil at gen.mpib-tuebingen.mpg.de)
FML der Max Planck Gesellschaft,
More information about the Yeast