Southern and labeling of oligos
TCHORZ at BIOTOP.UMCS.LUBLIN.PL
Mon Aug 26 05:57:34 EST 1996
I am looking for some advice concerning probe preparation
and southern hybridization.
I have 23-mer, moreover degree of degeneration is 64, so which
approach is the best for oligonucleotide labeling, 3' or 5', isotopic or
Furthermore, which membranes, blocking solutions and buffers are on
the top and give the best performance in recent days.
Maybe you know some kits which can simplify my life?
Thanks a lot for your time and valuable suggestion !!!
University of Maria Curie-Sklodowska
Department of Molecular Biology
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