nuclear prep for S. pombe

Mark Flory mflory at
Fri Dec 13 14:52:04 EST 1996

I was wondering if anyone could suggest a good protocol for preparation 
of nuclei from S. pombe?  I am particularly interested in a method that 
would allow nuclear proteins to be immunoprecipitated from the resulting  

Many Thanks!
Mark Flory
mflory at
University of Washington
Department of Biochemistry
Seattle, WA 

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