Liquid B-gal assays???? How to?

mike at ncsu.edu mike at ncsu.edu
Thu Dec 12 12:11:33 EST 1996


I would appreciate your sharing your favorite protocol for liquid
B-gal assays with me.  I am using PJ69.4a for a 2-hybrid screen and
will potentially being doing many at one time (or hopefully a few).  I
have tried grinding the cells with glass beads and then incubating the
extract with X-gal and Z buffer but it didn't work out (have yet to
repeat this).  Just wondering if anyone knows of a good way to do
this.

Thanks,
Mike Faughn

Dept. Genetics, NCSU




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