Liquid B-gal assays???? How to?

S Forsburg forsburg at salk.edu
Mon Dec 16 02:27:00 EST 1996


mike at ncsu.edu wrote:
> 
> I would appreciate your sharing your favorite protocol for liquid
> B-gal assays with me. 

Try the combined works of Guarente (start with Meth Enzymol 101:181
(1983)).  You can use chloroform and SDS to permeabilize the cells,
ONPG as your substrate, and normalize to culture OD.  The other 
classic method uses protein assays to normalize.  Maybe the 
Guthrie and Fink book or the red book also have protocols?


susan
-- 
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S L Forsburg          forsburg at salk.edu
The Salk Institute    http://flosun.salk.edu/~forsburg
La Jolla, CA



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