Yeast stabs

Richard Brennan brennan at toxsparc.harvard.edu
Fri Dec 20 11:50:21 EST 1996


Long-term storage: Add 10% Glycerol to overnight cultures store at -80C. Do 
not snap-freeze.

Medium term: Can be stored as slants on YPD agar in airtight 1oz bottles
(room temp).

Short-term: Can be kept for several weeks streaked on YPD plates at 4C
(NB some genotypes don't store well like this, notably sod mutants).

Richard
brennan at toxsparc.harvard.edu

Rico Laage (rico.laage at urz.uni-heidelberg.de) wrote:
: Hi its me again,
: we need a protocol for storage of  yeast , what kinds of stabs are best
: (glycerol or DMSO)...etc

: please answer fast x-mas is coming
: Rico 
: -- 
: *********************************************************************************
:  Rico Laage
:  Neurobiology                                Voice  +49 (6221)54-4863  
:  Uni Heidelberg                             Fax                         
: 54-4496   
:  Im Neuenheimer Feld
: 345                                                                           			  

: Germany                                                                                                                       
:  World-Wide-Web:
:  http://server.nbio.uni-heidelberg.de/Neurobiology.html          
: **********************************************************************************



More information about the Yeast mailing list