Namjin Chung nc1 at acpub.duke.edu
Mon Dec 30 11:32:23 EST 1996

In article <009AD84C.E710E800.1 at vaxa.weeg.uiowa.edu>,
cmdrub at VAXA.WEEG.UIOWA.EDU wrote:

> Hi, netters,
> I heard that Kanamycin(r) can be used as selectable markers for targeted
> gene disruption in yeast.  Can anyone provide me more information about
> this technique and where I can obtain a plasmid with Kan gene.  Thanks!
> Bing

Dear Bing,

Please refer to "New heterologous modules for classical or pcr-based gene
disruptions in saccharomyces cerevisiae" Yeast (1994) 10, 1793-1808. which
describes a detailed method for your purpose.

Hope this helps.

Namjin Chung (Graduate Student)
Program in Molecular Cancer Biology
Duke University Medical Center, Box 3345
Durham, NC 27710 USA

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