PCR from yeast preps

u42272 at UIC.EDU u42272 at UIC.EDU
Mon Feb 5 16:52:35 EST 1996


I've recently tried to PCR directly from yeast plasmid preps using
zymolyase and was not successful. I've also tried cleaning up the DNA from
the plasmid preps using Qiaspin prep columns and was again unsuccessful. On
the other hand, when I used colony PCR to check E.coli colonies transformed
with the yeast plasmid prep, the PCR worked. Does anyone have experience
using yeast plasmid preps (zymolyase) directly with PCR? Thank you.





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