GAL4 and bZIP proteins in 2-hybrid
imd at RESUNIX.RI.SICKKIDS.ON.CA
Mon Feb 12 12:50:57 EST 1996
Dear yeast netters
A paper published last year (Sollerbrant et. al NAR 23(4):588) indicated
that the GAL4 DNA-binding domain can interact with the bZIP domain of
c-JUN. Given this data, one might expect that a set of common false
positives from any two-hybrid screen would include the yeast bZIP proteins
(GCN4, YAP1/SNQ3/PAR1/PDR4 and CAD1). (These types of false positives
should be weeded out of a screen when tested against other non-relevant
baits, but this may not always be true.) Also, this observation might
explain why the GAL4 DNA-binding domain has a low level of activating
ability by itself in the two hybrid system; this activity is usually
destroyed by fusing something to the DNA-binding domain.
Has anyone pulled any of these bZIP proteins out of a 2-hybrid screen? Are
there other false positives that are reproducibly pulled out of the screen?
I have already done a search of the yeast.net archives and came up with
nothing of relevance.
Send me your junk (reproducible) and I will post a summary.
Thanks for any replies
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