Yeast competent frozen

Barry Causier bmb6bc at
Tue Feb 20 12:40:15 EST 1996

Dear All,

Dr. Gietz's yeast transformation home page can be found at:

It is full of transformation techniques including a step-by-step
frozen competent cell method based on Dohmen et al.

Hope this of help,


(Manuel G. CLAROS) wrote:
>I have heard something about freezing and storing competent yeast cells.
>Is it true?
>Is there a method described? where?

<David humair at bota.> wrote:
Dear Manuel,
You can see the next article: Dohmen et al. (1991) Yeast 7: 691-692
I know that  Mr.Gietz (University of Manitoba, Canada) has a "trafo
homepage on the net" about several techniques to transform yeast.

Dr. Barry Causier,
Dept. Biochemistry & Mol. Biology,
University of Leeds,
Leeds LS2 9JT, U.K.

Tel: 0113 233 3162
Fax: 0113 233 3167
E-mail: bmb6bc at

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