PCR with oligos ds
Depto. Biologia Celular
bc2biced at lucano.uco.es
Wed Feb 21 10:30:27 EST 1996
I work cloning a mutated gene of yeast in E.Coli. The mutation's
plan use two PCR to obtain the altered gen. With the first PCR I obtain
the primer sense to the second PCR, but when I do the the PCR was'nt work.
I think that is a problem to use like a primer sense doubled stranded.
I have discarded the formation of secondary structures on the primer.
Someone know a possible reason of this problem?
Dept. of Cell Biology
Univ. of Cordoba
E-mail bc2biced at lucano.uco.es
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