Yeast plasmid isolation/ E. coli transformation

Tracie Matsumoto traciem at sage.cc.purdue.edu
Thu Feb 22 21:04:05 EST 1996


Hi,

Can anyone recommend a good procedure for isolating single copy plasmids from 
yeast and transforming the plasmid into E. coli?  I have tried the standard
procedure with the breaking buffer and glass beads and transforming E coli 
with limited success.  Only one or two colonies if I am lucky.

Thanks

traciem at sage.cc.purdue.edu




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