pombe genetics question

Colin Rasmussen colin at fungus.biochem.ualberta.ca
Fri Feb 23 21:11:28 EST 1996


Jannatipour Mehrdad wrote:
> 
> On Wed, 21 Feb 1996, Steve Ingram wrote:
> 
> > Date: Wed, 21 Feb 96 22:16:33 GMT
> > From: Steve Ingram <ingram at uthscsa.edu>
> > To: yeast at net.bio.net
> > Subject: pombe genetics question
> >
> > I am aware that gene disruptions in pombe are normally done in a diploid,
> > but is there any problem with performing the disruption in a haploid, then
> > crossing the disruption strain to a WT haploid, allowing the diploid to grow
> > under no selection conditions, then analyzing tetrads to be certain the
> > viability is OK and that any phenotypes segregate with the disruption?
> > Steve Ingram
> > Dept. of Biochemistry
> > UTHSCSA
> > San Antonio, Tx 78284-7760
> > USA
> >
> What if the disruption of yfg causes lethal phenotype?
>                                         Mehrdad Jannatipour
>                                         University of Montreal


You are screwed !



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