Star colonies.

Preston Garrison garrisonp at uthscsa.edu
Sun Feb 25 00:34:45 EST 1996


In Article <4gk7bn$h83 at mserv1.dl.ac.uk>, dperez at dbm.ulb.ac.be (David
Perez-Morga) wrote:
>        Hello everybody,
>        Is anybody aware of a star-like yeast colony morphology?.  Under
>the light microscope, the cells have an avoidal shape and seems that the
>cell wall is very thin; they look more like footballs.  Very frequently you 
>can see them as long chains as if cell division was afected somehow.
>        The strain is Y153 (S. Elledge's lab) and the effect seems to be
>triggered by a plasmid encoding a non-yeast protein, interacting with a G
>protein.
>        Thanks in advance for any help.
>        Cheers,
>
>
>                                                David.

Sounds like you may have activated the pseudo-hyphal growth mode. See refs 
below from G. Fink's lab - there are many more. Of course, be sure it's not 
a contaminant first.

Whitehead Institute for Biomedical Research, Massachusetts
Institute of Technology, Cambridge 02142.
 Title
Unipolar cell divisions in the yeast S. cerevisiae lead to
filamentous growth: regulation by starvation and RAS.
 Source
Cell.68(6):1077-90, 1992 Mar 20.

 Liu H.Styles CA.Fink GR.
 Institution
Whitehead Institute for Biomedical Research, Massachusetts
Institute of Technology, Cambridge 02142.
 Title
Elements of the yeast pheromone response pathway required
for filamentous growth of diploids.
 Source
Science.262(5140):1741-4, 1993 Dec 10.

Kron SJ.Styles CA.Fink GR.
 Institution
Whitehead Institute for Biomedical Research, Cambridge,
Massachusetts.
 Title
Symmetric cell division in pseudohyphae of the yeast
Saccharomyces cerevisiae.
 Source
Molecular Biology of the Cell.5(9):1003-22, 1994 Sep.

Preston Garrison
garrisonp at uthscsa.edu
Biochem. Dept.
Univ of Texas Health Sci Ctr
San Antonio, Tx 78284-7760 USA
210-567-3702



More information about the Yeast mailing list