Maximum 3-AT concentration?

Daniel Harkness harkness at
Tue Feb 27 18:38:26 EST 1996

Hello All, can anybody with any experience using 3-AT tell me what the 
maximum concentration can be regarding something like the Elledge yeast
two-hybrid selections?  Is it productive to use 100mM?

Any info/references would be greatly appreciated.

Dan Harkness
harkness at

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