site-directed mutagenesis

Eckhard Boles boles at bio1.bio.th-darmstadt.de
Thu Jan 4 02:44:31 EST 1996


We have recently published a rapid and highly efficient method for 
PCR-based site-directed mutagenesis with ds-DNA using only one new 
primer in Curr Genet (1995) 28: 197-198. It is a very easy and 
effective method which works very well. However, in this paper we 
describe the use of Taq-DNA polymerase. Instead, we now suggest to use a 
DNA polymerase with proof-reading activity but which does not degrade the 
primer (for example EUROGENTEC sells such a polymerase).

Best regards
Eckhard




Dr. Eckhard Boles
Institut fuer Mikrobiologie
Universitaet Duesseldorf
Germany





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