minimal lacZ with activity in yeast ?

Chuck Miller rellim at MAILHOST.TCS.TULANE.EDU
Thu Jan 4 17:52:11 EST 1996


The lacZ reporter vectors used in yeast systems frequently contain the
entire lacZ gene (ca. 3 kb). Is there any reason for using the full lacZ
sequence in yeast reporter plasmids? In other cases, i.e. lacZ fusion
proteins, only a portion of lacZ is used and this seems to work well. My
actual question is--- what is the minimal lacZ fragment that can function
(giving moderate to strong signals) in a reporter construct in yeast (not
one that produces a fusion protein, but simply a truncated lacZ protein)? 

Thanks,

Chuck

  
Dr. Charles A. Miller,  rellim at mailhost.tcs.tulane.edu 
Dept. Environmental Health Sciences 
Rm. 374, Center for Bioenvironmental Research
School of Public Health and Tropical Medicine
Tulane University Medical Center  
1430 Tulane Ave. Box SL29                 
New Orleans, LA 70112               
Ph. 504-585-6942, Fx. 504-585-6939              

                                                                        
                   






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