PCR from yeast genomic DNA
jds2 at ufcc.ufl.edu
Sun Jan 7 20:00:03 EST 1996
I need to PCR amplify two Saccharomyces genes of approx. 1000 bp,
neither of which contains an intron according to the sequence database.
Since I have never attempted PCR from yeast genomic DNA, I have some
questions. Is it possible to use the S. cerevisiae genomic DNA (> 100
kb) sold by Promega ($95 / 100 ug) for this purpose? My other simple
choice is the Clontech Quick cDNA ($310 / 20 reactions). Obviously, if
the Promega genomic DNA will work, I can find other uses for the $200
price difference :-). In addition, any hints or special tricks for
running these PCR reactions would be greatly appreciated.
Department of Chemistry
University of Florida
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