Enzyme assays and protease problems
ddeaguia at oeb.harvard.edu
Mon Jan 8 14:15:07 EST 1996
Hi all, I have a quick question...Has anyone ever had problems when
performing enzyme assays in S. cerevisiae (with enzymes from an other
organism) caused by proteases found naturally in yeast? What I mean is,
if I want to do some biochemical experiments in this yeast using an enzyme
found in my favorite organism, do I have to take any special precautions
to avoid any form of degredation or alteration of my protein in vivo
while being produced in yeast?
Thanx in advance!
Daniel De Aguiar
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