Enzyme assays and protease problems

Dan Deaguiar ddeaguia at oeb.harvard.edu
Mon Jan 8 14:15:07 EST 1996


Hi all, I have a quick question...Has anyone ever had problems when 
performing enzyme assays in S. cerevisiae (with enzymes from an other 
organism) caused by proteases found naturally in yeast? What I mean is, 
if I want to do some biochemical experiments in this yeast using  an enzyme 
found in my favorite organism, do I have to take any special precautions 
to avoid any form of degredation or alteration of my protein in vivo 
while being produced in yeast?

Thanx in advance!

Daniel De Aguiar



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