PCR from yeast genomic DNA

Michael Lichten lichten at helix.nih.gov
Tue Jan 9 09:49:18 EST 1996


In article <4cpqaj$2a2 at no-names.nerdc.ufl.edu>, Jon Stewart
<jds2 at ufcc.ufl.edu> wrote:

> I need to PCR amplify two Saccharomyces genes of approx. 1000 bp, 
> neither of which contains an intron according to the sequence database.  
> Since I have never attempted PCR from yeast genomic DNA, I have some 
> questions.  Is it possible to use the S. cerevisiae genomic DNA (> 100 
> kb) sold by Promega ($95 / 100 ug) for this purpose?  My other simple 
> choice is the Clontech Quick cDNA ($310 / 20 reactions).  Obviously, if 
> the Promega genomic DNA will work, I can find other uses for the $200 
> price difference :-).  In addition, any hints or special tricks for 
> running these PCR reactions would be greatly appreciated.
> 
> Jon Stewart
> Department of Chemistry
> University of Florida

Dear Jon,

The easiest thing to do is to get a Saccharomyces cerevisia colony from a
friend.  You can PCR directly from colonies; see the archives of this
newsgroup for a discussion of protocols.

-- 
Michael Lichten
lichten at helix.nih.gov



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