T7 transcription in yeast
rdlab at gandalf.bio.uci.edu
Wed Jan 10 13:25:04 EST 1996
In article <lagilb-090196143517 at yyqi1.monsanto.com> lagilb at ccmail.monsanto.com (Larry Gilbertson) writes:
>Has anyone ever expressed T7 RNA polymerase in yeast (Saccharomyces) and
>used it to turn on a gene with a T7 promoter?
Chen, W., S. Tabor, and K. Struhl. 1987. Distinguishing between mechanisms
of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase.
Cell 50: 1047-1055.
They use the T7 promoter + RNAP to drive his3 in yeast.
Martin Hoyt (R H Davis lab)
Mol. Biol. and Biochem.
Univ. of California, Irvine
mahoyt at uci.edu
More information about the Yeast