T7 transcription in yeast

rlab davis rdlab at gandalf.bio.uci.edu
Wed Jan 10 13:25:04 EST 1996


In article <lagilb-090196143517 at yyqi1.monsanto.com> lagilb at ccmail.monsanto.com (Larry Gilbertson) writes:

>Has anyone ever expressed T7 RNA polymerase in yeast (Saccharomyces) and
>used it to turn on a gene with a T7 promoter?

See:

Chen, W., S. Tabor, and K. Struhl.  1987.  Distinguishing between mechanisms 
of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase.
Cell 50: 1047-1055.

They use the T7 promoter + RNAP to drive his3 in yeast.

Martin Hoyt (R H Davis lab)
Mol. Biol. and Biochem.
Univ. of California, Irvine
mahoyt at uci.edu



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