problem in 2-hybrid screen?

Tien-Hsien Chang tchang at
Thu Jan 11 09:49:01 EST 1996

     A colleague of mine was told by others that a highly basic protein
used as a bait in the 2-hybrid screen will unavoidably yield numerous unwanted 
false positives, as it presumably will interact, nonspecifically, with many 
acidic proteins.  I wonder whether anyone know of this type of rumor (or truth) 
?  Thanks in advance for input in this matter.

               Tien-Hsien Chang
               Department of Molecular Genetics
               The Ohio State University

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