Yeast Vector Selectable Marker

Michael Lichten lichten at helix.nih.gov
Tue Jan 16 08:45:39 EST 1996


In article <4de5ii$mg at internal-dns.clontech.com>, Ying Luo
<yluo at clontech.com> wrote:

> In addition to TRP, LEU, HIS, URA, LYS, I need to choose something else. 
> Dose anyone have experience with ARG or other markers for selection? 
> Thanks!
> 
> Ying Luo
> Palo Alto, CA 

I have plasmids with various arg4 markers that all are welcome to, along
with plasmids for 2-step integration, one-step disruption, etc.  

You should be aware of the fact that can1 (canavanine resistance) is
incompatible with arginine auxotrophy, since CAN1 encodes the arginine
permease.

-- 
Michael Lichten
lichten at helix.nih.gov



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