Those tiny bubbles.....(in YPD plates)

Arnoud Kal kal at eland.amc.uva.nl
Thu Jan 18 13:12:08 EST 1996


On Wed, 17 Jan 1996, Michael Lichten wrote:

> I'm a bit embarassed to post this, as one would think I would have solved
> this one by now....
> 
> Recent batches of my YPD plates have many tiny bubbles in them, or what
> looks like tiny bubbles in the tetrad dissecting scope.  These bubbles are
> just a little bit smaller than a spore, so you can well imagine how much
> fun dissecting tetrads is on these plates.  To make matters worse, I KNOW
> that I solved this problem 10 years ago, but I can't for the life of me
> remember what the solution was.

I heard or read that some batches of agar give plates that are not 
cristal clear, so you might poure a few plates from a different source or 
try agarose (expensive plates!).

Good luck!

Arnoud.

Arnoud Kal
Department of Biochemistry
University of Amsterdam
Academic Medical Centre
Meibergdreef 15
1105 AZ Amsterdam
tel. +31-20-5665381/5665382
fax. +31-20-6915519




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