TaKaRa Aureobasidin "
toddlorenz at ghawk.com
Fri Jan 19 03:42:00 EST 1996
-=> Quoting Arnoud Kal to All <=-
AK> A week ago I posted a request for information about the TaKaRa
AK> Aureobasidin selection system. The only replies I received were from
AK> others who were also interested in experiences of yeast colleagues
AK> with this system, but who do not use the system themselves.
AK> Apparently this selection system is too new and none of us has tried
AK> it.... Maybe it is better if I re-post the question in a few months.
Arnoud Kal...I saw the ad also and called for more info. They sent me
a fax the same afternoon. If I remember correctly each of the
plasmids are $500...and get this, the aureobasidin is $500 per
mg....*outrageous* price by anyones standards. C'mon TaKaRa get
real no one is going to pay even close to that. The tech info
says that the MIC is about 0.5-1 ug/ml so thats 1-2 liters of
media to select, basically one experiment with a clone bank.
They say they are running a limited time special that you can
get both plasmids
and 1 mg of their proprietary antifungal for $500. But
remember, once you buy into their system you are stuck paying
exorbitant prices for the selection each time. I would not
use this system unless they drastically lower the price of the
aureobasidin, competitive with hygromycin. It is almost worth
developing a good Saccharomyces vector with hygromycin
resistance, you can get it from Sigma for a reasonable price.
BTW...Does anyone have a Saccharomyces shuttle vector (E.coli)
with a hygromycin r gene, drop me a note please...
<Todd Lorenz....IGENE Biotechnology, Columbia MD>
Senior Sci-guy, making fish pinker and healthier
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